Express-diagnosis of the diseases of the eye and its appendages by scanning electron microscopy with supravital staining

Novikov IA, Kravchik MV, Pak OA, Kasparova EA, Yartsev VD, Rodina ES, Solodovnikov VI, Subbot AM. Express-diagnosis of the diseases of the eye and its appendages by scanning electron microscopy with supravital staining. Russian Annals of Ophthalmology. 2023;139(3‑2):136‑144. (In Russ.)
https://doi.org/10.17116/oftalma2023139032136

Доказательная гастроэнтерология: pro et contra

Актуальные вопросы педиатрической практики

Использование инфографики авторами научных работ

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Proteomic analysis in microbiology. Molecular Genetics, Microbiology and Virology. 2023;(1):3-9

This review describes the history of development of a new line of chemical reagents that prompts to significantly reevaluate the application of scanning electron microscopy (SEM) in medical and biological studies, particularly in ophthalmology; considers the establishing of SEM as an analytical method; covers the problems in its application associated with the needs of clinical medicine and the complexities of biological sample preparation for electron microscopy. The article also presents in chronological order the technical solutions associated with creating a unique line of reagents for supravital staining. The multitude of technical solutions allows considering SEM as a method of express diagnostics. The review discusses examples of practical application of these methods for solving certain cases in clinical ophthalmology. The niche of SEM is considered among other methods of clinical diagnostics, as well as its future development involving the use of artificial intelligence.

Keywords:

scanning electron microscopy (SEM)

lanthanides

microbiology

microbiome of the eye

Authors:

I.A. Novikov

Krasnov Research Institute of Eye Diseases

ORCID: 0000-0003-4898-4662

M.V. Kravchik

M.M. Krasnov Research Institute of Eye Diseases

ORCID: 0000-0001-5764-4198

O.A. Pak

Krasnov Research Institute of Eye Diseases

ORCID: 0000-0003-4682-0795

E.A. Kasparova

Krasnov Research Institute of Eye Diseases

ORCID: 0000-0001-8594-2395

V.D. Yartsev

Krasnov Research Institute of Eye Diseases

ORCID: 0000-0003-2990-8111

E.S. Rodina

Sechenov First Moscow State Medical University

ORCID: 0000-0002-5525-1793

V.I. Solodovnikov

Center of Information Technologies in Design of the Russian Academy of Sciences

ORCID: 0000-0001-5533-214X

A.M. Subbot

M.M. Krasnov Research Institute of Eye Diseases

ORCID: 0000-0002-8258-6011

Received:

21.02.2023

Accepted:

25.02.2023

List of references:

Avetisov ES, Andreeva LD, Khoroshilova-Maslova IP. Elektronno-mikroskopicheskoe izuchenie sklery glaza cheloveka v raznykh vozrastnykh gruppakh. Vestnik oftal’mologii. 1979;1:24-30. (In Russ.).
Stroeva OG, Poplinskaya VA, Khoroshilova-Maslova IP, Panova IG. Elektronno-mikroskopicheskoe i morfometricheskoe issledovanie dejstviya preparata ENKAD na fotoretseptornye kletki setchatki u krys Campbell s nasledstvennoj distrofiej setchatki. Vestnik eksperimental’noj biologii i meditsiny. 1990;109(5):504-506. (In Russ.).
Fedorov AA. Morphological basics of scientific research in ophthalmology. Vestnik oftal’mologii. 2013;129(5):10-21. (In Russ.).
Mamikonyan VR, Voevodina TM, Fedorov AA, Budzinskaya MV, Balayan ML. Experimental and morphological study of the effect of antiangiogenic therapy on corneal neovessels. Vestnik oftal’mologii. 2013;129(6):45-50. (In Russ.).
Fedoruk NA, Fedorov AA, Bolshunov AV. Morphological and histochemical effects of subthreshold laser therapy on the chorioretinal complex. Vestnik oftal’mologii. 2013;129(5):74-82. (In Russ.).
Novikov IA, Subbot AM, Pak OA, Chebotar IV. A two-step labeling of biological object’s ultrastructures by neodymium and lead for scanning electron microscopy. Analytics. 2018;(4):358-363. (In Russ.). https://doi.org/10.22184/2227-572X.2018.41.4.358.363
Swift JA, Brown AC. An environmental cell for the examination of wet biological specimens at atmospheric pressure by transmission scanning electron microscopy. J Phys E. 1970;3(11):924-926. https://doi.org/10.1088/0022-3735/3/11/426
Parsons DF, Matricardi VR, Moretz RC, Turner JN. Electron microscopy and diffraction of wet unstained and unfixed biological objects. Adv Biol Med Phys. 1974;15(0):161-270. https://doi.org/10.1016/b978-0-12-005215-8.50012-7
Kravchik MV, Novikov IA. Classification of chemical elements in biomedical research. Analytics. 2021;11(3):234-239. (In Russ.). https://doi.org/10.22184/2227-572x.2021.11.3.234.239
Novikov IA, Subbot AM, Kiryushchenkova NP, et al. Fast and easy method of lanthanoid staining for visualization of cellular ultrastructure and spatial arrangement. AIP Conference Proceedings. 2016: AIP Publishing LLC. https://doi.org/10.1063/1.4954343
Novikov IA, Subbot AM, Fedorov AA, Griboedova IG, Antonov EN, Vakhrushev IV. Supravital lanthanoid staining for scanning electron microscopy of biological objects. Genes & Cells. 2015;10(2):90-96. (In Russ.).
Chebotar IV, Novikov IA, Subbot AM, Mayansky NA. Lanthanoid staining as a fast technology of preparing microbiological specimens for scanning electron microscopy. Sovremennye tekhnologii v meditsine. 2017;9(3):23-30. (In Russ.). https://doi.org/10.17691/stm2017.9.3.03
Novikov IA, Vakhrushev IV, Antonov EN, Yarygin KN, Subbot AM. Visualization of mesenchymal stromal cells in 2D and 3D-cultures by scanning electron microscopy with lanthannoid staining. Bulletin of Experimental Biology and Medicine. 2016;4:248-253. (In Russ.). https://doi.org/10.1007/s10517-017-3659-4
Novikov IA, Subbot AM, Trufanov SV, Tekeeva LY. A new look on the ultrastructure of the cell adhesion complex of the corneal epithelium. Point of View. East-West. 2017;1:64-66. (In Russ.).
Novikov I, Subbot A, Turenok A, Mayanskiy N, Chebotar I. A rapid method of whole cell sample preparation for scanning electron microscopy using neodymium chloride. Micron. 2019;124:102687. https://doi.org/10.1016/j.micron.2019.102687
Avetisov SE, Trufanov SV, Novikov IA, Subbot AM, Fedorov AA. SEM visualization of corneal epithelium through lanthanoid staining based on Ca/Nd isomorphous substitution in Ca-dependent molecular systems. Vestnik oftal’mologii. 2016;132(6):11-19. (In Russ.). https://doi.org/10.17116/oftalma2016132611-19
Kravchik MV, Rodina ES, Subbot AM, Pimonova OI, Fettser EI, Novikov IA. Visualization of normal ocular surface microflora via impression cytology sample using scanning electron microscopy with lanthanide contrasting. Vestnik oftal’mologii. 2022;138(6):5-13. (In Russ.). https://doi.org/10.17116/oftalma20221380615
Khalatyan AS, Pimonova OI, Subbot AM, Novikov IA. Rapid test of eye surface microbiota visualization. Sovremennye tekhnologii v oftal’mologii. 2020;4:264-265. (In Russ.). https://doi.org/10.25276/2312-4911-2020-4-264-265
Yartsev V, Zolotenkova G, Kasparova E, Kislov M, Rodina E, Novikov I, Atkova E. Ocular microbiome features in patients with chronic alcoholism. Abstractband DOG 2022. Die Ophthalmologie. 2022;119(Suppl 3):287. https://doi.org/10.1007/s00347-022-01723-2
Rodina ES, Chebotar IV, Kravchik MV, Lyamin AV, Fettser EI, Zaitsev AV, Novikov IA. Creating a reference atlas of cultures for evaluation of microbiological status of ocular surface by scanning electron microscopy with lanthanide contrasting. Sovremennye tekhnologii v oftal’mologii. 2022;3:241-247. (In Russ.). https://doi.org/10.25276/2312-4911-2022-3-241-247
Gridin VN, Novikov IA, Salem BR, Solodovnikov VI. Poluavtomaticheskaya razmetka odnoklassovykh izobrazhenij s primeneniem nejrosetevoj modeli obnaruzheniya ob”ektov. Informatsionnye tekhnologii i nanotekhnologii (ITNT-2022): sbornik trudov po materialam VIII Mezhdunarodnoj konferentsii i molodezhnoj shkoly (Samara, 23—27 maya 2022 goda): v 5 tomakh. (In Russ.).

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